RNA Details
Disease Name
pulmonary arterial hypertension
Tissue
lung
RNA Symbol
Akt1
RNA ID
RNA Type
mRNA
Alteration Pattern
histone deacetylation
Species
rattus norvegicus
Detection Methods
Immunofluorescence; Immunoprecipitation assay etc.
Target
NA
Pathway
NA
PubMed ID
Title
Sodium butyrate inhibits platelet-derived growth factor-induced proliferation and migration in pulmonary artery smooth muscle cells through Akt inhibition
Year
2013
Function
"In PASMCs of PAH rats, Sodium butyrate (BU) counteracted platelet-derived growth factor (PDGF)-induced Ki67 expression, and arrested the cell cycle, mainly at G0 /G1 . BU decreased proliferating cell nuclear antigen, c-Myc and cyclin D1 transcription and protein expression, while increasing p21 expression. BU reduced the transcription of PDGF receptor-B, and that of Ednra and Ednrb, two major receptors in PAH progression. Wound healing, migration and pulmonary artery ring assays indicated that BU inhibited PDGF-induced PASMC migration. BU strongly inhibited PDGF-induced Akt phosphorylation, an effect reversed by the phosphatase inhibitor calyculin A. BU-treated cells showed a remarkable increase in acetylated Akt, indicating an inverse relationship between the levels of acetylated Akt and phospho-Akt."